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Inverted Metallurgical Microscope ECLIPSE MA200 Instructions M498E 08.10.NF.1 (2/4)

Contents 8 3.13.2 Adjusting the polarizer direction ...

5Troubleshooting 98 Improper use of the microscope may adversely affect performance, even if the microscope is not damaged. If any of the problems

Chapter 5 Troubleshooting 99 Problem Cause Countermeasure Dirt or dust is seen in the view-field. The aperture diaphragm is stopped down too far.

Chapter 5 Troubleshooting 100 Problem Cause Countermeasure The lamp voltage is too low. Adjust the brightness with the brightness control dial.

Chapter 5 Troubleshooting 101 Problem Cause Countermeasure A wrong objective is used. Place an appropriate objective into the optical path. (p.3

6Care and Maintenance 102 Nikon recommends daily care and maintenance for maintaining the performance as long as possible. Do not let dust, fingerp

Chapter 6 Care and Maintenance 103 6.3 Storage • Store this product in a dry place where mold is not likely to form. • Store the objectives and

7Specifications 104 Type ECLIPSE MA200 Dimension 413 (W) × 337 (D) × 308 (H) mm, including the handle and mount parts Weight 20 kg Optical system

Chapter 7 Specifications 105 Power cord When used in 100-120 V region, outside Japan: UL listed detachable power cord set, 3 conductor grounding (3

Contents 9 4.3 Network Connection...

1Part Name and Function 10 Figure 1.1-1 Front view, Right-side view Eyepiece tube (Figure illustrates the MA2-TI3.) Binocular eye

Chapter 1 Part Name and Function 11 Figure 1.1-2 Front view Display POWER LED Displays ON/OFF for the power switch or the light-status

Chapter 1 Part Name and Function 12 Figure 1.1-3 Left view, Rear view Binocular tube Vertical tube Back port Supporting Pilla

Chapter 1 Part Name and Function 13 Figure 1.1-4 Connectors on the rear About product parts names in this manual The right colu

2Microscopy 14 This chapter describes the procedure of each microscopy. See Table 2.1 for the items required for each microscopy. • Refer to “4. A

Chapter 2 Microscopy 15 Table 2.1 Items required for the microscopy Microscopy Page Illuminator Revolving nosepiece Objective Other items req

Chapter 2 Microscopy 16 2.1 Bright-field Microscopy under the Episcopic Illumination Turn on the power to light up the episcopic illumination la

Chapter 2 Microscopy 17 Set the microscope for the bright-field microscopy under the episcopic illumination. If the current unit is the one shown i

Chapter 2 Microscopy 18 Set the desired magnification and observe the sample. 1 Turn the revolving nosepiece to place the objective of a desired m

Chapter 2 Microscopy 19 2.2 Dark-field Microscopy under the Episcopic Illumination Attach the items required for the dark-field microscopy under

Chapter 2 Microscopy 20 2.3 Polarization Microscopy under the Episcopic Illumination (simplified/sensitive color) Attach the items required for t

Chapter 2 Microscopy 21 2.4 Differential Interference Contrast Microscopy under the Episcopic Illumination Attach the items required for the diff

Chapter 2 Microscopy 22 Return to the bright-field microscopy under the episcopic illumination. 1 Pull the polarizer/analyzer unit to the first

Chapter 2 Microscopy 23 Return to the bright-field or dark-field microscopy under the episcopic illumination. 1 Turn the BD field changeover lev

Chapter 2 Microscopy 24 2.6 Bright-field Microscopy under the Diascopic Illumination Attach the items required for the bright-field microscopy u

Chapter 2 Microscopy 25 Set the microscope for the bright-field microscopy under the diascopic illumination. When the “Other items required” (See t

Chapter 2 Microscopy 26 Place the sample onto the stage to adjust the focus. 1 Use the sample holder according to the selected sample. Place th

Chapter 2 Microscopy 27 Center the condenser. (See 3.23.2.) 1 Be sure that the objective is set to the 10X objective. If not, turn the revolv

1 Introduction Thank you for purchasing a Nikon product. This instruction manual is written for users of Nikon Inverted Metallurgical Microscope E

Chapter 2 Microscopy 28 2.7 Polarization Microscopy under the Diascopic Illumination (simplified/sensitive color) Attach the items required for t

3Operation Details 29 3.1 Power ON/OFF 3.1.1 Power of the microscope Figure 3.1-1 Power of the microscope The power switch for the product

Chapter 3 Operation Details 30 3.2 Illumination 3.2.1 Brightness control and illumination ON/OFF Figure 3.2-1 Brightness control and illumina

Chapter 3 Operation Details 31 3.2.3 Displaying the POWER LED Figure 3.2-3 Displaying the POWER LED The status of the illumination lamp app

Chapter 3 Operation Details 32 Table 3.3-1 Selecting the microscopy method Microscopy Revolving nosepiece Objective Items required for the opti

Chapter 3 Operation Details 33 3.4 Eyepiece Tube 3.4.1 Selecting optical path • The light distribution for both the binocular and vertical tube

Chapter 3 Operation Details 34 3.4.2 Adjusting the eyelevel risers Eyelevel risers can be used to adjust the height of the eyepiece tube for the us

Chapter 3 Operation Details 35 3.6 Adjusting the Diopters Adjust the diopter according to the user's eyesight using the diopter adjustment r

Chapter 3 Operation Details 36 3.7 Adjusting the Focus (for focus operation) 3.7.1 Using the coarse/fine focus knob Focus on the sample lifting/

Chapter 3 Operation Details 37 3.8 Placing the Sample and Operating the Stage 3.8.1 Placing the sample Figure 3.8-1 Placing the sample Nor

2 Safety Precautions To ensure correct and safe operation, read this manual before using the product. Warning and Caution Symbols Used in This Manua

Chapter 3 Operation Details 38 3.8.2 Changing the Observation Position The rectangular stage is equipped with the knob to move the stage in all fo

Chapter 3 Operation Details 39 3.9 Operating the Revolving Nosepiece and the Objective 3.9.1 Revolving nosepiece in combination with the objecti

Chapter 3 Operation Details 40 3.10 Filter Figure 3.10-1 Filter turret Two quartet filter turrets are provided on the microscope, and each filt

Chapter 3 Operation Details 41 3.11 Adjusting the Field Diaphragm (for the episcopic Illumination) Figure 3.11-1 Field Diaphragm Adjustment

Chapter 3 Operation Details 42 3.12 Adjusting the Aperture Diaphragm (for the episcopic illumination) Figure 3.12-1 Aperture diaphragm adj

Chapter 3 Operation Details 43 Adjustment with centering telescope (C-CT) Remove one of the eyepieces and attach the centering telescope instead.

Chapter 3 Operation Details 44 3.13.2 Adjusting the polarizer direction Each time you turn the polarizer rotation ring, the direction of the polar

Chapter 3 Operation Details 45 3.14 Using the λ Plate (MA2-λP) Figure 3.14-1 λ plate slider Attach the λ plate to the polarizer/analyzer u

Chapter 3 Operation Details 46 3.15 Using the DIC Slider (L-DIHC/L-DIC) Figure 3.15-1 L-DIHC/L-DIC slider Attach the L-DIHC/L-DIC DIC sl

Chapter 3 Operation Details 47 3.15.3 Interference color You can change the interference color continuously by turning the prism movement knob. Inte

Safety Precautions 3 WARNING 1. Intended product use The product should only be used for microscopic observation. Do not use this microscope for o

Chapter 3 Operation Details 48 3.16.3 Interference color You can change the interference color continuously by adjusting the orientation of the pol

Chapter 3 Operation Details 49 3.18 Using the λ Plate (D-LP) Figure 3.18-1 λ plate slider Attach the D-LP λ plate to the sextuple revolvin

Chapter 3 Operation Details 50 3.19 Using the Fluorescent Unit (MA2-FL) Attach the fluorescent unit to the MA200 main body to perform the epi-fl

Chapter 3 Operation Details 51 3.19.2 Excitation light filter (EX filter) An excitation light filter transmits lights selectively and blocks oth

Chapter 3 Operation Details 52 3.19.3 Barrier filter (BA filter) A barrier filter transmits only fluorescent lights emitted by the sample but bloc

Chapter 3 Operation Details 53 3.20 Using the Scale Slider (MA2-GR/MA2-MR) Attach the MA2-GR/MA2-MR slider to the MA200 main body to measure stee

Chapter 3 Operation Details 54 3.20.2 Scale slider (MA2-MR) Figure 3.20-2 Scale slider Three items of reticles including “magnification,” “length

Chapter 3 Operation Details 55 3.21 Using the Intermediate Magnification Unit (MA2-MC) Figure 3.21-1 Intermediate magnification unit Attach

Chapter 3 Operation Details 56 3.22 Using the HG Precentered Fiber Illuminator (C-HGFI/C-HGFIE) Figure 3.22-1 HG Precentered Fiber Illuminat

Chapter 3 Operation Details 57 3.23 Using the supporting pillar for dia-Illuminator 100W (MA2-DP) Attach the supporting pillar for dia-Illuminato

Safety Precautions 4 7. Heat from the light source The lamp and the lamphouse become extremely hot. To avoid burns, do not touch the lamphouse whil

Chapter 3 Operation Details 58 3.23.2 Focusing and centering the condenser To perform the diascopic illumination for the first time or to replace t

Chapter 3 Operation Details 59 3.23.3 Adjusting the field diaphragm (for the diascopic illumination) The field diaphragm is used to limit the irrad

Chapter 3 Operation Details 60 3.23.4 Adjusting the aperture diaphragm (for the diascopic illumination) Adjust the size of the aperture diaphra

Chapter 3 Operation Details 61 Filter list Filter Description ND2 (Neutral Density filter) Adjusts the brightness for general microscopy or photomi

Chapter 3 Operation Details 62 3.23.8 Polarizer slider (T-P2), λ plate (TI-DIC) When performing the simplified polarization microscopy under the di

Chapter 3 Operation Details 63 Dark cross that appears in the objective pupil Figure 3.23-10 Adjusting the polarizer direction 7 Loosen the c

Chapter 3 Operation Details 64 3.24 Using the Supporting Arm (MA2-MP) The supporting arm is designed to mount Nikon camera control unit DS-L2. Th

Chapter 3 Operation Details 65 3.25 Using the DS Camera Control Unit (DS-L2) Once you connect the DS Camera Control Unit DS-L2 with the DS camera

Chapter 3 Operation Details 66 Figure 3.25-2 Objective info Displaying the objective info Press the button, and the name and other info are dis

Chapter 3 Operation Details 67 3.25.5 Connecting with the DS camera head switcher (DS-SW: Camera switch BOX, optional) If the DS-L2 is connected wi

Safety Precautions 5 CAUTION 1. Handle with care This product is a precision optical instrument. Handle the microscope system with care to avoid sh

4Assembly 68 WARNING • Before assembling the microscope, be sure to read the WARNING and CAUTION at the beginning of this instruction ma

Chapter 4 Assembly 69 Combination of the illuminator and the light source Use this product with a specified light source. The specified light so

Chapter 4 Assembly 70 4.1 About the System D-LP λ plate slider D-DA analyzer slider Eyepiece D-ND6 revolving nosepiece D-NI7 revolving nosepi

Chapter 4 Assembly 71 4.2 Combination List for the Unit Combinations for each microscopy provided for MA200. : All selectable or available. c: S

Chapter 4 Assembly 72 1: For EPI objective on 5 revo., “LU nosepiece adapter M32-25” is required. 2: BD objective is unavailable on 6 revo., 7 rev

Chapter 4 Assembly 73 4.3 Network Connection 4.3.1 Unit system capable of establishing network The table below shows a unit system capable of es

Chapter 4 Assembly 74 4.3.2 Viewing user control options and status displays on a PC or the DS-L2 The table below shows user control options and s

Chapter 4 Assembly 75 Table 4.3-1 User control options for the unit using the PC/DS-L2 PC DS-L2 Unit Control Status Control Status Brightness

Chapter 4 Assembly 76 4.3.3 Registering information on the objective You can register the information to be checked on a PC or the DS-L2 with the “

Chapter 4 Assembly 77 4.4 The LV-LH50PC Lamphouse and the Lamp CAUTION for heat Do not touch the lamp and the lamphouse while the lamp is o

Safety Precautions 6 • Do not hold the focus knobs, eyepiece tube, lamphouse, stage, and so on, when carrying the microscope. They may come off and

Chapter 4 Assembly 78 4.4.2 Replacing the lamp 1 Loosen the lamphouse cover clamp screw using the supplied hexagonal wrench. 2 Remove the lamph

Chapter 4 Assembly 79 4.5.2 Cable connection for the manual revolving nosepiece and the MA200 Connect the cable of the revolving nosepiece to the c

Chapter 4 Assembly 80 4.6 Stage • To attach or remove the stage, hold the stage securely to prevent from being fallen. • To remove the stage f

Chapter 4 Assembly 81 4.7 Objectives 1 Remove the sample holder from the stage. 2 Screw the objectives into the socket of the revolving nosepi

Chapter 4 Assembly 82 4.10 Grain Scale Slider/Scale Slider 1 Remove the dust protection cover from the scale slider slot on the bottom-front of t

Chapter 4 Assembly 83 4.12 Various Sliders for Attaching the Revolving Nosepiece To insert a slider, be sure to attach the objective in advance.

Chapter 4 Assembly 84 4.12.2 LV-DIC/LV-DIHC slider (Senarmont method) Attach the DIC slider in Senarmont method to the D-ND6 revolving nosepiece.

Chapter 4 Assembly 85 4.13 Polarizer/analyzer Unit and Fluorescent Unit 1 Loosen the front-cover fixing bolt with the supplied hexagonal screwdr

Chapter 4 Assembly 86 4.14 Intermediate Magnification Unit Opening for the intermediate magnification unit 1 Remove four bolts to take out th

Chapter 4 Assembly 87 4.15 External Light Source You can attach the HG precentered fiber illuminator (for the episcopic illumination) or the supp

7 Contents Introduction ...

Chapter 4 Assembly 88 * For the C-HGFIE motorized fiber illuminator, connect the C-HGFIE and the [HGFIE] connector on the MA200 using the RS232C cr

Chapter 4 Assembly 89 Attach the condenser to the condenser mount. You can attach the TI-C condenser turret (system condenser) or ELWD-S condenser

Chapter 4 Assembly 90 Attaching the ELWD-S condenser With the indication on the turret facing the front (towards the user), insert the conden

Chapter 4 Assembly 91 Attach/replace the lamp. Be sure to use the halogen lamp (OSRAM HLX64623 or PHILIPS77241) exclusive for the D-LH/LC Lamphouse

Chapter 4 Assembly 92 Attach the filter and the slider. • Do not touch filters or other optical components with your bare hands. • Latches at bo

Chapter 4 Assembly 93 Attach the T-P2 polarizer slider. 1 Remove the system condenser, if attached, from the condenser holder to attach the

Chapter 4 Assembly 94 4.16 Supporting Arm (for DS-L2) You can mount the camera control unit DS-L2 on the MA200 main body by attaching the support

Chapter 4 Assembly 95 Attaching the DS-L2 1 Remove the one-touch release attached to the front of the mount arm for the supporting arm. Pull

Chapter 4 Assembly 96 4.17 Eyelevel Risers 1 Fully loosen the eyepiece tube clamp screw on the microscope, to mount the eyelevel riser onto the

Chapter 4 Assembly 97 4.18 Camera Adapter You can perform photomicroscopy by mounting the photomicrographic equipment such as CCD camera on the m